An In Vitro HL-1 Cardiomyocyte-Based Olfactory Biosensor for Olfr558-Inhibited Efficiency Detection

Some short-chain fatty acids with a pungent or unpleasant odor are important components of human body odor. These malodors severely threaten health. The antagonists would help to improve perception by affecting the interaction between odors and their receptors. However, traditional detection analysis methods, such as MOS, electrochemical, conductive polymer gas sensors, chromatography-mass spectrometry not suitable for screening since they unable detect ligand efficacy after odor-receptor binding. In this study, RT-PCR results showed that HL-1 cardiomyocytes endogenously express olfactory receptor 558 (Olfr558) which can be activated several malodorous acids. Therefore, an in vitro cardiomyocyte-based biosensor (HCBO-biosensor) was developed combining microelectrode array (MEA) chips potential Olfr558. Firstly, it specifically responded ligands Olfr558 through stimulation experiments. Then, response model constructed (isovaleric acid). feature HCBO-biosensor individual mixtures antagonist (citral β-damascenone) were extracted compared. Finally, Olfr558-inhibited efficiency indirectly detected comparing half-maximal inhibitory concentration isovaleric acid. β-damascenone greatly inhibited while citral no significant effect. conclusion, we built novel method based on MEA chip will assist odor-related companies develop